INTERPRETATION OF BACTERIOLOGICAL AND PCR RESULTS IN THE DETECTION OF SALMONELLA SPP.

• Iroda Xalilla qizi Sobirova

  Author

This literature-based review examines the principles for interpreting bacteriological and PCR results. Differences in the analytical targets, control status, and assessment of discordant findings are summarized.

1. GOST 31659-2024 (ISO 6579-1:2017). Microbiology of the food chain — Horizontal method for the detection, enumeration and serotyping of bacteria of the genus Salmonella — Part 1: Detection of Salmonella spp. Sections 4, 9, and 10.

2. Malorny B., Hoorfar J., Bunge C., Helmuth R. Multicenter Validation of the Analytical Accuracy of Salmonella PCR: towards an International Standard. Applied and Environmental Microbiology. 2003;69(1):290–296. DOI: 10.1128/AEM.69.1.290-296.2003.

3. Uyttendaele M., Vanwildemeersch K., Debevere J. Evaluation of real-time PCR vs automated ELISA and a conventional culture method using a semi-solid medium for detection of Salmonella. Letters in Applied Microbiology. 2003;37(5):386–391. DOI: 10.1046/j.1472-765x.2003.01415.x.

4. FDA. Guidance for Industry: Testing for Salmonella Species in Human Foods and Direct-Human-Contact Animal Foods. March 2012.

5. Rashidovna, F. V., & Saloxiddinovna, R. S. (2022). Innovative approaches to preschool education management. International Journal of Early Childhood Special Education, 14(4).

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