QUALITY CONTROL AND STANDARDIZATION OF BIOLOGICALLY ACTIVE SUBSTANCES INCLUDED IN THE COMPOSITION OF THE DRY EXTRACT 'EXTRADENT'

• Shohidahon Saitovna Yuldasheva

  Author

• Dilbarxon Alisher qizi Akhmadjonova

  Author

The present study is devoted to the quality control and standardization of the dry extract 'Extradent', a phytopreparation composed of Calendula officinalis, Urtica dioica, Capsella bursa-pastoris, and Polygonum hydropiper. These plants are rich in flavonoids, carotenoids, tannins, amino acids, and mineral elements, which collectively determine the wide pharmacological spectrum of the extract. In this research, high-performance liquid chromatography (HPLC) and UV-spectrophotometry were applied to evaluate the chemical composition with a special focus on flavonoids. Rutin was identified as the principal marker compound in the chromatogram, with a significant retention peak, thus confirming its suitability for analytical standardization. Complementary UV-spectrophotometric measurements demonstrated the presence of a broad spectrum of flavonoids beyond rutin, contributing to the biological activity of the preparation. Literature data were also considered to outline the role of other biologically active substances, such as carotenoids, tannins, amino acids, and mineral elements, in enhancing therapeutic properties. The findings highlight that combining chromatographic and spectrophotometric methods provides a reliable approach to ensure reproducibility, safety, and compliance with international standards. This study may serve as a basis for developing methodological guidelines for the certification and wider application of the 'Extradent' extract in preventive and therapeutic medicine.

1. World Health Organization. WHO Traditional Medicine Strategy 2014–2023. Geneva: WHO, 2013.

2. Middleton E, Kandaswami C, Theoharides TC. The effects of plant flavonoids on mammalian cells: implications for inflammation, heart disease, and cancer. Pharmacol Rev. 2000;52(4):673–751.

3. Pietta PG. Flavonoids as antioxidants. J Nat Prod. 2000;63(7):1035–1042.

4. Harborne JB, Williams CA. Advances in flavonoid research since 1992. Phytochemistry. 2000;55(6):481–504.

5. Kumar S, Pandey AK. Chemistry and biological activities of flavonoids: an overview. Sci World J. 2013;2013:162750.

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