OKSIDLOVCHI STRESS SHAROITIDA JIGARNING DETOKSIFIKATSIYA TIZIMIDAGI BIOKIMYOVIY O'ZGARISHLAR: FERMENTATIV FAOLLIK VA ANTIOKSIDANT HIMOYA MEXANIZMLARI

• Nurmuhammad Xusanboy o'g'li Mamazulunov

  Author

• Muhammadzoxid Xasanboy o'g'li Dehqonov

  Author

Ushbu tadqiqot oksidlovchi stress sharoitida jigar detoksifikatsiya tizimining fermentativ faolligida yuz beradigan biokimyoviy o'zgarishlarni eksperimental jigar modellari asosida tahlil qiladi. Malondialdegid (MDA), qaytarilgan glutationning (GSH), superoksid dismutaza (SOD), katalaza, glutationperoksidaza (GPx), sitoxrom P450 izofermentlari (CYP1A2, CYP2E1, CYP3A4), glyutation-S-transferaza (GST) va UDP-glyukuronoziltransferaza (UGT1A1) ko'rsatkichlari spektrofotometrik usulda aniqlandi. Natijalar oksidlovchi stress kuchayishi bilan I faza fermentlari faolligining ikki bosqichli — avval kompensator (30–45%) ortishi, so'ngra keskin (58–65%) pasayishi kuzatilishini ko'rsatdi. II faza fermentlari faolligida dastlabki bosqichdayoq sezilarli pasayish qayd etildi (GST 69%, UGT1A1 71% gacha). GSH miqdori 72% ga kamaydi, MDA esa 6,2 baravar oshdi. Pearson korrelyatsiya tahlili MDA bilan GSH o'rtasida kuchli teskari korrelyatsiya (r = −0,92; p < 0,001) borligini tasdiqladi. Tadqiqot natijalari jigar patologiyalarida oksidlovchi stress va detoksifikatsiya tizimi o'rtasidagi patobiokimyoviy o'zaro bog'liqlikni yoritib, klinik jihatdan muhim antioksidant strategiyalarni asoslashga zamin hozirlaydi.

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